Zeitschriftenaufsätze

Background - The surface of nanoparticles (NPs) is an important factor affecting the process of poly/peptides' amyloid aggregation. We have investigated the in vitro effect of trisodium citrate (TC), gum arabic (GA) and citric acid (CA) surface-modified magnetite nanoparticles (COAT-MNPs) on hen egg-white lysozyme (HEWL) amyloid fibrillization and mature HEWL fibrils. - Methods - Dynamic light scattering (DLS) was used to characterize the physico-chemical properties of studied COAT-MNPs and determine the adsorption potential of their surface towards HEWL. The anti-amyloid properties were studied using thioflavin T (ThT) and tryptophan (Trp) intrinsic fluorescence assays, and atomic force microscopy (AFM). The morphology of amyloid aggregates was analyzed using Gwyddion software. The cytotoxicity of COAT-MNPs was determined utilizing Trypan blue (TB) assay. - Results - Agents used for surface modification affect the COAT-MNPs physico-chemical properties and modulate their anti-amyloid potential. The results from ThT and intrinsic fluorescence showed that the inhibitory activities result from the more favorable interactions of COAT-MNPs with early pre-amyloid species, presumably reducing nuclei and oligomers formation necessary for amyloid fibrillization. COAT-MNPs also possess destroying potential, which is presumably caused by the interaction with hydrophobic residues of the fibrils, resulting in the interruption of an interface between [beta]-sheets stabilizing the amyloid fibrils. - Conclusion - COAT-MNPs were able to inhibit HEWL fibrillization and destroy mature fibrils with different efficacy depending on their properties, TC-MNPs being the most potent nanoparticles. - General significance - The study reports findings regarding the general impact of nanoparticles' surface modifications on the amyloid aggregation of proteins.

Driven by the strong demand for sparsity in dimensional reduction techniques, a sparse modeling and monitoring approach based on sparse, distributed principal component analysis (SDPCA) is proposed to achieve sparsity. To this end, the data set is first divided into highly correlated blocks (HCBs) and one remainder block (RB) on the basis of the mutual-information-based correlation matrix. From this, the sparse loading vectors for the HCBs are obtained using the PCA models, while for the RB, it is obtained using the sparse PCA model. It is worth noting that the sparsity in SDPCA enables the sparse loading vectors to produce interpretable principal components, which keeps the correlations between the highly correlated variables and achieves the sparsity for the weakly correlated ones. Moreover, to fully appreciate the interpretation of the sparse principal components, a fault diagnosis strategy named blockwise contribution plots is proposed by first determining the faulty block, and then, identifying the faulty variables. Compared with PCA and SPCA, the proposed SDPCA detects more faulty samples and gives more accurate diagnosis results.

In recent years, the interest of the scientific community in perforated plates for ballistic protection has increased. Perforated plates do not represent protection by themselves, rather, they are used in the armour systems of armoured vehicles, in conjunction with base armour, since they are intended to induce bend stresses, where a penetrating core fracture occurs. The fragments are subsequently stopped by base armoured vehicle armour. Although for the first time used several decades ago, perforated plates are found to be attractive even today. The main reason is the combination of very convenient properties. Besides high mass effectiveness, they possess a high multi-impact resistance, since their perforations arrest cracks. Therefore, a relatively wide array of materials is suitable for perforated plate fabrication, ranging from alloy steel to some types of cast iron. Being made of metallic materials, raw material costs are relatively low compared to ceramics or composite materials, making them very attractive for present and future armoured vehicles. Finally, armour system consisting of a perforated plate and base plate at some distance, reduce the effectiveness of both shaped charge jets and act as blast mitigators.

In ultrasonic non-destructive testing, array and matrix transducers are being employed for applications that require in-field steerability or which benefit from a higher number of insonification angles. Having many transmit channels, on the other hand, increases the measurement time and renders the use of array transducers unfeasible for many applications. In the literature, methods for reducing the number of required channels compared to the full matrix capture scheme have been proposed. Conventionally, these are based on choosing the aperture that is as wide as possible. In this publication, we investigate a scenario from the field of pipe inspection, where cracks have to be detected in specific areas near the weld. Consequently, the width of the aperture has to be chosen according to the region of interest at hand. On the basis of ray-tracing simulations which incorporate a model of the transducer directivity and beam spread at the interface, we derive application specific measures of the energy distribution over the array configuration for given regions of interest. These are used to determine feasible subsampling schemes. For the given scenario, the validity/quality of the derived subsampling schemes are compared on the basis of reconstructions using the conventional total focusing method as well as sparsity driven-reconstructions using the Fast Iterative Shrinkage-Thresholding Algorithm. The results can be used to effectively improve the measurement time for the given application without notable loss in defect detectability.