Publikationen

Acoustic tweezers facilitate a noninvasive, contactless, and label-free method for the precise manipulation of micro objects, including biological cells. Although cells are exposed to mechanical and thermal stress, acoustic tweezers are usually considered as biocompatible. Here, we present a holistic experimental approach to reveal the correlation between acoustic fields, acoustophoretic motion and heating effects of particles induced by an acoustic tweezer setup. The system is based on surface acoustic waves and was characterized by applying laser Doppler vibrometry, astigmatism particle tracking velocimetry and luminescence lifetime imaging. In situ measurements with high spatial and temporal resolution reveal a three-dimensional particle patterning coinciding with the experimentally assisted numerical result of the acoustic radiation force distribution. In addition, a considerable and rapid heating up to 55 ˚C depending on specific parameters was observed. Although these temperatures may be harmful to living cells, counter-measures can be found as the time scales of patterning and heating are shown to be different.

By integrating surface acoustic waves (SAW) into microfluidic devices, microparticle systems can be fractionated precisely in flexible and easily scalable Lab-on-a-Chip platforms. The widely adopted driving mechanism behind this principle is the acoustic radiation force, which depends on the size and acoustic properties of the suspended particles. Superimposed fluid motion caused by the acoustic streaming effect can further manipulate particle trajectories and might have a negative influence on the fractionation result. A characterization of the crucial parameters that affect the pattern and scaling of the acoustically induced flow is thus essential for the design of acoustofluidic separation systems. For the first time, the fluid flow induced by pseudo-standing acoustic wave fields with a wavelength much smaller than the width of the confined microchannel is experimentally revealed in detail, using quantitative three-dimensional measurements of all three velocity components (3D3C). In Part I of this study, we focus on the fluid flow close to the center of the surface acoustic wave field, while in Part II the outer regions with strong acoustic gradients are investigated. By systematic variations of the SAW-wavelength λSAW and channel height H, a transition from vortex pairs extending over the entire channel width W to periodic flows resembling the pseudo-standing wave field is revealed. An adaptation of the electrical power, however, only affects the velocity scaling. Based on the experimental data, a validated numerical model was developed in which critical material parameters and boundary conditions were systematically adjusted. Considering a Navier slip length at the substrate-fluid interface, the simulations provide a strong agreement with the measured velocity data over a large frequency range and enable an energetic consideration of the first and second-order fields. Based on the results of this study, critical parameters were identified for the particle size as well as for channel height and width. Progress for the research on SAW-based separation systems is obtained not only by these findings but also by providing all experimental velocity data to allow for further developments on other sites.

Continuous wetting of a surface with liquid metal is indispensable in many applications, such as in fusion reactors. In the present study, we provide data on the suppression of free-surface instabilities of liquid metal film flows under the action of strong streamwise magnetic fields in analogy to the poloidal fields used in application. We have designed and built up an experimental test setup which allows studying the influence of magnetohydrodynamics on the dynamic behaviour of liquid metal GaInSn film flows in laminar, transient, and turbulent regimes. While the width and the length of the film are adjusted at w = 23 mm and l = 120 mm, respectively, we are able to apply strong uniform magnetic fields up to B = 5 T over the entire fluid-flow volume. Moreover, the setup allows to vary the Reynolds number within the range 200 ≤ Re ≤ 1700. The corresponding Hartmann and Stuart numbers are Ha ≤ 180 and N ≤ 40, respectively. This study shows that a streamwise magnetic field is capable of suppressing free-surface instabilities even in the turbulent regime of the film flow by dampening any motion perpendicular to the applied magnetic field. Plans for future studies include the quantitative investigation of the parameter space.

For the transition of our energy supply towards a higher share of renewables, thermal energy storage (TES) systems are, besides electric batteries and chemical energy storage systems, one promising solution to overcome the volatile nature of renewable energy sources. For the most efficient operation, the liquid storage material in the tank should be stratified by its temperature-dependent density. As a result, the cold fluid remains at the bottom, and the heated fluid rises to the top (Alva et al. (2018)). Typically steel tanks are used for TES, and thus, the wall material has a thermal diffusivity that is one to two orders of magnitude higher than that of the storage fluid. Consequently, the tank’s sidewalls work as a thermal bridge between the stratified layers. In recent studies, the authors have shown that the resulting heat flux induces two counterdirected, convective wall jets near the sidewalls of the tank, which increase mixing of the stratification and thus lowers the exergy content and the storage efficiency (Otto et al. (2019, 2020)). Using a model experiment of a TES, the entire vertical extent of the detected wall jets is investigated. Hence, the typical flow structures of vertical, natural convection under the influence of non-zero temperature gradients in the ambient fluid can be analyzed, which can help to improve storage tanks in the future. The velocity in the region of the wall jets is measured via 2d particle-image velocimetry (PIV) in a rectangular model experiment of 750 mm height on a base area of 375 mm × 375 mm made from polycarbonate. The jets evolve on the surface of an aluminum plate simulating the storage tank’s sidewall. The measuring system consists of four cameras with a resolution of 2160 × 2560 pixels combined with objective lenses with 100 mm focal length capturing the raw images in a plane perpendicular to the aluminum wall. A Nd:YAG laser with a wavelength of 532 nm illuminates the measuring plane. Simultaneously using up to four cameras adjacent to each other and stitching their resulting vector fields, the vertical extent of the field of view increases from 38 mm up to 140 mm. Despite this, the field of view is still much smaller than the vertical extent of the model experiment, so that seven consecutive runs are performed to cover the entire height. Disturbing reflections of the laser light sheet on the aluminum wall are eliminated using optical filters for the cameras that are opaque for the green laser light in combination with fluorescently (Rhodamine B) dyed PMMA tracer particles with a diameter between 1–20 μm. The particles emit light at a wavelength of 610 nm (orange light) and can therefore be detected through the cameras’ filters. During four separate measuring periods, where each lasts for two minutes, double frame images are captured with a time difference of 19.981 ms (maximum possible value) at a measuring frequency of 7 Hz. Figure 1 shows a schematic of the camera setup next to the model experiment and the measurement and evaluation procedure to finally receive one time-averaged velocity field per measuring period of the full height of the experiment. The raw data evaluation process starts with calculating the vector fields of all cameras used at a certain measuring position and stitching them to one flow field of this position. Since the wall jets’ horizontal extents are with 2–7 mm relatively small and they show high velocity gradients, the raw images are evaluated in both single-frame and double-frame mode. With a velocity threshold that corresponds to a pixel displacement of 1/4 of the interrogation window size and the time difference of the single-frames, the resulting vector fields are masked and merged into one final vector field. This vector field consists of high velocities evaluated in double-frame mode and low velocities evaluated in single-frame mode (see Figure 2) thus minimizing the relative error. The algorithm used in this work is similar to the multi-frame PIV approach introduced by Hain and Kähler (2007). Figure 3 shows the time-averaged results of the first measuring period for each of the seven measuring positions in height.

The application of standing surface acoustic waves (sSAW) has enabled the development of many flexible and easily scalable concepts for the fractionation of particle solutions in the field of microfluidic lab-ona-chip devices. In this context, the acoustic radiation force (ARF) is often employed for the targeted manipulation of particle trajectories, whereas acoustically induced flows complicate efficient fractionation in many systems [Sehgal and Kirby (2017)]. Therefore, a characterization of the superimposed fluid motion is essential for the design of such devices. The present work focuses on a structural analysis of the acousticallyexcited flow, both in the center and in the outer regions of the standing wave field. For this, experimental flow measurements were conducted using astigmatism particle tracking velocimetry (APTV) [Cierpka et al. (2010)]. Through multiple approaches, we address the specific challenges for reliable velocity measurements in sSAW due to limited optical access, the influence of the ARF on particle motion, and regions of particle depletion caused by multiple pressure nodes along the channel width and height. Variations in frequency, channel geometry, and electrical power allow for conclusions to be drawn on the formation of a complex, three-dimensional vortex structure at the beginning and end of the sSAW.